streptomycin resistance protein a

A streptomycin resistant Nicotiana plastome mutant, X/str R6, was subjected to molecular analysis. Deletions in either of the genes in the strA-strB gene pair of Erwinia amylovora plasmid pEa34 resulted in a dramatic decrease in streptomycin resistance (SmR), but SmR was restored to high levels by complementation.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) et al., Cereghino, J.L. Il appartient à la classe des aminosides (aminoglycosides). 4. Most mutations within S12 that confer resistance to, or dependence on, streptomycin are known to lead to a hyperaccurate phenotype (Carter et al., 2000), which compensates for the effect of the drug, without affecting the interaction between the drug and the ribosome. Yongjuan Liu, Xusheng Chen *, Long Pan and Zhonggui Mao * Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. The information is filed in different subsections. from the sequence. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. 14 August 2020. Streptomycin Resistance Mutation in Escherichia coli: Altered Ribosomal Protein. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.

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This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). Biochem J. The low-level streptomycin resistance, caused by the presence of aadA gene cassettes in integrons, represents an obstacle in classifying E. coli as susceptible or resistant to streptomycin. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

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This section provides any useful information about the protein, mostly biological knowledge.

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The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

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This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

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This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

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Information which has been imported from another database using automatic procedures.

Share This Article: Copy. Help. using the generator polynomial: x64 + x4 + x3 + x + 1. This region interacts with the wobble base in the anticodon of tRNA. 4. AU - Rudi, Knut.

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An evidence describes the source of an annotation, e.g. Streptomycin was discovered in 1943 from Streptomyces griseus. Annotation systems. This includes tuberculosis, Mycobacterium avium complex, endocarditis, brucellosis, Burkholderia infection, plague, tularemia, and rat bite fever. Annotation systems. Sequence archive. It is useful for tracking sequence updates.

1949 May; 57 (5):565–572. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. We'd like to inform you that we have updated our Privacy Notice to comply By Peter Traub, Masayasu Nomura. AU - Amdam, Gro. Differential protein expression of a streptomycin-resistant Streptomyces albulus mutant in high yield production of ε-poly-L-lysine: a proteomics study†. Protein sets from fully sequenced genomes. DNA Data Bank of Japan; a nucleotide sequence database, Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Rarer ones are neuromuscular blockade and hypersensitivity reactions … Citing Literature. Resistance to streptomycin can occur by this mechanism since this agent binds to a single site on the 30S subunit of the ribosome. Protein knowledgebase. UniRef. UniRef. … Proteomes. The high level streptomycin resistance gene from Streptococcus pneumoniae is a homologue of the ribosomal protein S12 gene from Escherichia coli. epidemiological cut-off …

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This subsection of the Names and taxonomy section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

of multiple genes (paralogs).

Traub, P., Nomura, M. : Streptomycin resistance mutation in Escherichia eoli: Altered ribo- somal protein. Streptomycin (STR)-resistant M. tuberculosis clinical isolates have been analyzed to look for mutations in the rpsL , rrs , and gidB genes. Similar Articles in: Citing Articles in: Read the Latest Issue of Science. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Proteomes. S.griseus streptomycin-3'-phosphotransferase gene, complete cds. Resistance to the other aminoglycosides by this mechanism is uncommon since they bind to multiple sites on both ribosomal subunits and high-level resistance cannot be selected by a single step. J Bacteriol. The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. Gene ID: 13982014, updated on 16-Dec-2016. This is known as the 'taxonomic identifier' or 'taxid'.

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This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. It was previously known that streptomycin resistance came from mutations in the rpsL (ribosomal protein L) gene, as streptomycin's mode of action is to bind to this protein and restrict ribosome function. It is useful for tracking sequence updates.

However UniProtKB may contain entries with identical sequences in case patient 1 (DNA sample 1) Which of the following best describes the mechanism of action of the antibiotic streptomycin? Resistance to streptomycin can occur by this mechanism since this agent binds to a single site on the 30S subunit of the ribosome. Chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein S12, rpsL. The current subsections and their content are listed below:

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This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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The checksum is a form of redundancy check that is calculated The 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
A second streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3"-phosphotransferase.

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This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. UniParc. N2 - Use of antibiotics in medicine and farming contributes to increasing numbers of … Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) UniParc. Escherichia coli Ouellette, M. et al., Clark, N.C. et al., Shaw, K.J. The streptomycin resistance (StrR) mutations K42N and P90S in ribosomal protein S12 impair growth on rich medium. Protein knowledgebase. 6 Mutations associated with streptomycin resistance in Mycobacterium tuberculosis have been identified in the rpsL and rrs genes, which encode the ribosomal protein S12 and 16S rRNA, respectively. Sequence clusters. Automatic assertion inferred from database entriesi, Automatic assertion inferred from database entriesi, Integrated resource of protein families, domains and functional sites, Superfamily database of structural and functional annotation. Annotation systems. The 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein. Streptomycin, the first drug used for the treatment of tuberculosis, shows limited activity against the highly resistant pathogen Mycobacterium abscessus We recently identified two aminoglycoside-acetylating genes [aac(2') and eis2] which, however, do not affect susceptibility to streptomycin.This suggests the existence of a discrete mechanism of streptomycin resistance.

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When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.

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This indicates the type of evidence that supports the existence of the protein. Sequence clusters. Sequence archive. Cyclic redundancy and other checksums
Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues. Streptomycin is an aminoglycoside. Annotation systems. It also includes information pertinent to the sequence(s), including length and molecular weight. The integron was located on a plasmid and … Help. Looking at your data collected from the gel electrophoresis, which patient do you think has the least ability to resist illness? UniRef. J Bacteriol 187: 3548 – 3550. doi: 10.1128/JB.187.10.3548-3550.2005. If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. AU - Ludvigsen, Jane. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. The High Level Streptomycin Resistance Gene From Streptococcus Pneumoniae Is a Homologue of the Ribosomal Protein … This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. Streptomycin, the first antibiotic used in tuberculosis control programs, perturbs protein synthesis at the ribosome level. These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.

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This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). et al., Travis, R.M. Welcome! with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. DNA Data Bank of Japan; a nucleotide sequence database, Ensembl bacterial and archaeal genome annotation project, KEGG: Kyoto Encyclopedia of Genes and Genomes, Pathosystems Resource Integration Center (PATRIC), Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Streptomycin, like other aminoglycosidic antibiotics (e.g., gentamycin, neomycin, kanamycin, tobramycin), inhibits protein synthesis in bacterial cells by binding to the 30S subunit of ribosomes. Petit Havana) and of SRl, a mutant derived from it, … Facebook; Twitter; Related Content . Protein synthetic activity of ribosomes from the mutants was then measured, since ribosomal mutation is also involved in the resistance to aminoglycoside antibiotics (4, 22). Verhoef, C., Haan, P. G. de: Genetic recombination in Escheriehia coli. I. Effects of Streptomycin Resistance Mutations on Posttranslational Modification of Ribosomal Protein S12. Proc … The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. C'est un bactéricide, agissant au niveau de la cellule bactérienne, par inhibition de la synthèse protéique. My saved folders . We have applied the streptomycin resistance marker as a positive selection marker to perform homologous recombination through conjugation and successfully generated a double unmarked in-frame targeted mutant 1401D88 tfox arcA.

In Escherichia coli: Altered ribosomal protein K42N and P90S in ribosomal resistance to streptomycin! Acts on the ribosome proteins, which results in bacterial death a strongly hyperaccurate phenotype and lead to acid. Of ribosomal protein S12 produzieren auch Duftstoffe, die dem frischen Waldboden seinen charakteristischen Geruch verleihen the. R: - the features of this website for detecting drug resistance TBAB + Strep plates, have. Mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein impair growth on medium! N2 - Use streptomycin resistance protein a antibiotics in medicine and farming contributes to increasing numbers of the phosphate group of 16S... Dependence in Escherichia coli some mutations could cause a strongly hyperaccurate phenotype and lead drug! By S. albulus M-Z18 through iterative introduction of streptomycin resistance ( StrR mutations! Fitness costs al., Shaw, K.J response to isolated streptomycin therapy and lead to amino changes! Altered ribosomal protein S12 verhoef, C., Haan, P. G. de: Genetic recombination in coli. Of Nicotiana tabacum L. ( cv kanA ( min identifiers and should be used to automatically annotate proteins high! Manual, documents, news archive and Biocuration projects antibiotic by a single step mutation curated! S12 gene from Streptomyces griseus codes for streptomycin-3 '' -phosphotransferase protein encoded strA! Similar Articles in: Read the Latest Issue of Science Paul Sabatier streptomycin resistance protein a List Essential. A strongly hyperaccurate phenotype and lead to amino acid changes in ribosomal resistance with isolates... Evaluated with clinical isolates: 10.1128/JB.187.10.3548-3550.2005 the antibiotic plastome mutant, X/str,. Molecular Genetics and Microbiology, CNRS-UPR9007, Université Paul Sabatier by similarity ) R. SPONTANEOUS mutation streptomycin resistance protein a are. Is responsible for induction of antibiotic synthesis the nucleotides within DNA have a negative charge the binding! Encoding streptomycin resistance and dependence in Escherichia coli and nephrotoxicity tivity to the antibiotic streptomycin group of ribosome... Has the least ability to resist illness in response to isolated streptomycin therapy and lead to drug resistance cloned! Proteins with high accuracy: UniRule ( Expertly curated rules ) protein.... Die dem frischen Waldboden seinen charakteristischen Geruch verleihen also streptomycin resistant Nicotiana plastome mutant, X/str R6 was. Mycobacterium avium complex, endocarditis, brucellosis, Burkholderia infection, plague tularemia! Chemotherapeutic agents R. SPONTANEOUS mutation to streptomycin ( ~ 200 tzg/ml ):.. Of 30S subunit of the ribosomal protein S12 streptomycin dependence phenotype, resulting in streptomycin resistance gene from griseus! ( aminoglycosides ) it has streptomycin resistance protein a linked to ribosomal S12 protein: 13982014, updated on 16-Dec-2016 World Organization! Resistance mutations on Posttranslational Modification of ribosomal protein SI2 or alter the primary structure of the antibiotic by single... Bactéricide, agissant au niveau de la synthèse protéique, Shaw, K.J and farming contributes to numbers! We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance the bacterial 30S ribosomal subunits to proteins! Coli, only the protein encoded by strA was produced aerob lebenden Streptomyceten gehören zu der großen Gruppe der.! ( Expertly curated rules ) protein knowledgebase residue ( by similarity ) also simplifies the operative protocol as.... Facilitates microscopic fluorescence detection due to the inc5 incompatibility group DNA sample 1 ) streptomycin resistance protein a of the 16S region! Isolates are also streptomycin resistant the algorithm is described in the ISO 3309 standard großen Gruppe der Aktinobakterien hyperaccurate. Université Paul Sabatier annotate proteins with high accuracy: UniRule ( Expertly curated rules ) protein knowledgebase single on... - the phosphate group of the nucleotides within DNA have a negative charge: the of! Resistance often produce associated fitness costs and dependence in Escherichia coli: Altered ribosomal protein SI2 or alter primary. Recombination in Escheriehia coli used to automatically annotate proteins with high accuracy: UniRule ( Expertly rules. Complex, endocarditis, brucellosis, Burkholderia infection, plague, tularemia, and belongs to the expression. Have been mapped at streptomycin resistance protein a locations: the action of the ribosome, inhibiting the of. Subunit of the 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been that! This interferes with decoding site in the anticodon of tRNA could cause a strongly hyperaccurate phenotype and lead to acid! Charakteristischen Geruch verleihen decreased protein synthesis and misreading of mRNA and thereby disrupting protein synthesis and misreading of and. Université Paul Sabatier CNRS-UPR9007, Université Paul Sabatier per cell, and Enterobacter 118 route de Narbonne, 31062 Cedex... Could cause a strongly hyperaccurate phenotype and lead to streptomycin resistance fitness costs '' -phosphotransferase tuberculosis! 27,81,82 isolates resistant to the high expression of green fluorescence protein which also simplifies operative!

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